Isolation of β-Amyrin from Plant Parts of M. barteri

Isolation of ÃŽ ²-Amyrin from Plant Parts of M. barteri ISOLATION AND BIOLOGICAL ACTIVITY OF THE TRITERPENE Î’-AMYRIN FROM THE AERIAL PLANT PARTS OF MAESOBOTRYABARTERI(BAILL). Abstract Maesobotrya  barteri(Baill), belonging to the family EUPHORBIACEAE, is a medicinal plant growing widely in tropical Africa. The Aerial plant parts of Maesobotrya barteri (Bail) were collected fresh from Orokam, Ogbadibo local Government of Benue State, Nigeria in July, 2013. Taxonomical identification was done by Mallam Musa Abdullahi at the Herbarium unit of Biological sciences Department, ABU, Zaria, Nigeria. Pulverized aerial parts of Maesobotrya  barteri (960g) was exhaustively extracted successively using petroleum ether, chloroform, ethylacetate and methanol and concentrated in the rotary evaporator at 40oC. The ethylacetate fraction having the highest activity against test microbes from preliminary crude microbial screenings was subjected to phytochemical studies, antimicrobial analysis and column chromatography (CC). The column chromatography yielded fraction EN, which was further purified using preparative thin layer chromatography to give EN1. The structure of the isola ted compound was established using 1-D NMR and 2-D NMR spectroscopic analysis and by direct comparism with data reported in literature was confirmed to be ÃŽ ²-amyrin. The bioactivity of this compound was carried out using some clinical pathogens and the activity compared with standard drugs and this was found to be comparable with the standard drug. Keywords: Maesobotrya  barteri; Medicinal plant; bioactivity; Ethylacetate extract; ÃŽ ²-amyrin Introduction Maesobotrya sp is a variety of flowering plant belonging to the family Phyllanthaceae, or by some authors classified in Euphorbiaceae. The Euphorbiaceae plants are shrubs, trees, herbs or rarely lianas [1]. Plants of the Euphorbiaceae are known to be rich in terpenoids (69.5%) [2]. In Nigeria, the species M.barteri is under-exploited although the tree is of both medicinal and nutritional importance [3]. Maesobotrya species are used medicinally in different regions in Africa [4]. It bears succulent black-purple fruits that are edible and stain the tongue. The nutritive values of the fruits and seeds have been studied in Southern Nigeria [5]. Thus, this study aims at validating the antimicrobial effects of the aerial plant parts of Maesobotrya barteri used in traditional medicine in Orokam town of Benue State,Nigeria, as well as potent compounds that can be used as precursors for synthetic drugs. The presence of secondary metabolites in plants generally has been confirmed by [6], [7] and [8] through useful procedures and suggestions to get to targeted precursors which are of useful aids in the formulation of modern drugs. Plant Material The aerial parts of Maesobotrya barteri were collected from Orokam in Ogbadibo local government area of Benue State in the month of July, 2013. They were properly identified at the herbarium, Department of Biological Sciences, Ahmadu Bello University, Zaria. The whole plant was sorted, air dried under shade, segregated and pulverized by mechanically pounding them using wooden mortar and pestle. The pulverized plant material was stored away from moisture. Extraction 980.54g of the pulverized plant materials were carefully weighed and loaded into a Soxhlet extractor. It was extracted successively with Petroleum ether (60-80oC), Chloroform, Ethyl acetate (76-78oC) and Methanol by hot continuous percolation method in the Soxhlet apparatus for 72 hours respectively. Solvents used were those of JHD and general purpose reagents The extracts were concentrated in vacuo at 40oC using rotary evaporator and subjected to air drying to give dried crude extracts. Isolation The ethyl acetate extract was the most sensitive extract from the antimicrobial screening and was subjected to column chromatography (CC) for fractionation. Solvents used to run the column chromatograghy were sealed and are products JHD with 98% purity. Fifteen grams (15g) of the extract was dissolved in ethyl acetate and preadsorbed on 10.0g silica gel (qualikens 60-120 mesh). The dried pre-adsorbed extract was transferred to a mortar and ground to give a fine powder and was added at the uniform layer on top of the column. The petroleum ether descended on a horizontal line indicating that the column was well packed. A total of 105 fractions (50mls) each were initially collected using gradient elution with a solvent combination of petroleum ether and ethyl acetate, starting with 100% petroleum ether with an increasing polarity of 1% ethyl acetate. Similar fractions were pulled together based on monitoring from TLC. Fractions F20-F25 were pulled together at the ration of 8:2 and where subjected to further purification using Preparative thin layer chromatography to obtain a solid white amorphous substance which after several trails using different solvent combinations showed a single spot which was visible under the UV lamp and after spraying the TLC plate with 10% H2SO4 and oven dried for 5minutes at 60oC. The solid white amorphous substance produced was insoluble in ethyl acetate or petroleum ether but soluble in chloroform. Results and Discussion From the preliminary antimicrobial screening of the crude aerial plant parts extracts of M. barteri, ethylacetate extract exhibited the highest activity against the test microbes used via their zones of inhibition. Therefore, an activity guided isolation was undertaken. Bioactivity of Compound EN1 Pure Isolate The antimicrobial activities of compound EN1 isolated from ethylacetate extract of the aerial plant parts of M. barteri was examined and agar disc diffusion method [13] was employed for the determination of the antimicrobial activities. The pure compound was determined using some pathogenic microbes; the microbes were obtained from the department of Medical Microbiology Ahmadu Bello University Teaching- Hospital, Zaria, Nigeria. The determination of minimum inhibitory concentration was carried out using the nutrient broth susceptibility assay prepared according to the manufacturer’s instructions, as recommended by NCCLS [14]. Minimum inhibition McFarland turbidity standard scale number 0.5 was prepared to give turbid solution. Normal saline was prepared and was dispensed into test tube and the test microorganism was then inoculated into the normal saline, incubation was at 37oC for 6hrs, dilution of the microorganism in the normal was performed until the turbidity marched that of the McFarland by visual comparison at this point the microorganism had a concentration of about 1.5à Ã‚ ¥108 cfu/ml. Minimum Bactericidal and fungicidal concentration of compound EN1 was carried out to check whether the test microbes were killed or only their growth was inhibited. Mueller Hunton agars were prepared according to the manufacturer’s instruction, as recommended by NCCLS [14]. They were boiled to dissolve and were sterilized at 121oC for 15 minutes, the media were cool to 45oC and the medium (20ml) was poured in to sterile Petri dishes, the plates were covered and allowed to cool and solidify. The contents of the MIC in the serial dilution was inoculated on to the media, the media were incubated at 37oC for 24hrs for the bacteria and at 30oC for 1-7 days for fungi, after which the plate were observed for colonies growth. The MBC/MFC was the plate with lowest concentrations of the extract without colony growth. Table 2 shows the Zones of inhibition (mm) of the pure the compound EN1 from the ethyl acetate fraction which showed remarkable activity against twelve of the sixteen organisms tested. Compound EN1 could not inhibit the growth of Corynebacterium ulcerans, Candida albicans, Candida tropicalis, Aspergillus fumigates, Aspergillus nigre. The various minimum inhibitory concentration (MIC) and minimum Bacteriocidal concentration and fungicidal concentration (MBC/MFC) for the different microbes are as shown in Tables and the bioactivity of the aerial plant parts of M. barteri is comparable to the drugs Ciprofloxacin, Fluconazole and Fulcin used as positive controls. Table 2: Zone of inhibition of Compound EN1 against the test microorganism Test Organism Compound EN1 Ciprofloxacin Fluconazole Fulcin Staphylococus aureus 32 37 0 0 Streptococus pyogenes 30 35 0 0 Streptococcus feacalis 32 39 0 0 Corynebacteruim ulcerans 0 32 0 0 Escherichia coli 32 38 0 0 Klebsiella pneumonia 32 40 0 0 Salmonella typhi 30 42 0 0 Shigella dysenteriae 30 40 0 0 Candida albicans 0 0 35 0 Candida krusei 27 0 37 0 Candida tropicalis 0 0 32 0 Candida stellatoidea 25 0 37 0 Microsporum sp 26 0 0 38 Aspargillus fumigates 0 0 0 32 Aspargillus nigre 0 0 0 34 Trichophyton rutarum 28 0 0 38 Table 2 and 3 shows the activity of the compound EN1 which the spectroscopic analysis was proposed to be a ÃŽ ²-Amyrin or ÃŽ ²-Amyrenol, (C30H50O, 426.7 g/mol). Antimicrobial screening reported from other natural products has also confirmed the microbial properties of ÃŽ ²-Amyrin. ÃŽ ²-Amyrin was isolated from Ardisia elliptica [9], a medicinal plant used for alleviating chest pain, fever, liver poisoning and parturition complications. It was found that ÃŽ ²-Amyrin was six times as active as aspirin in inhibiting platelets aggregation. ÃŽ ²-amyrin was isolated for the first time fromLaurencia microcladia, marine algaedistributed widely in Egypt found to have antibacterial activity against Staphylococcus aureus, Bacillus subtilis, Salmonella typhi, Escherichia coli, and Pseudomonas aeurginosa [10]. Table 3a: Minimum Inhibition Concentration (MIC) Test Organisms 3.12 µg/ml 6.2 µg/ml 12.5 µg/ml 25 µg/ml 50 µg/ml Concentration Staphylococus Aureus o* + Streptococus Pyogenes o* + + Streptococcus Feacalis o* + Escherichia Coli o* + Klebsiella Pneumonia o* + Salmonella Typhi o* + Shigella Dysenteria o* + Candida Krusei o* + + Candida Stellatoidea o* + + Microsporum Sp o* + + Trichophyton Rubrum o* + + KEY: = No turbidity (No growth), o* = MIC, + = Turbid (Growth) Table 3b: Minimum Bactericidal Concentration and Minimum Fungicidal Concentration (MBC/MFC) Test Organisms 3.12 µg/ml 6.2 µg/ml 12.5 µg/ml 25 µg/ml 50 µg/ml Concentration Staphylococus aureus o* + + + Streptococus pyogenes o* + + + Streptococcus feacalis o* + + + Escherichia coli o* + + + Klebsiella pneumonia o* + + Salmonella typhi o* + + + Shigella dysenteria o* + + + Candida krusei o* + + + Candida stellatoidea o* + + + + Microsporum sp o* + + + + Trichophyton rubrum o* + + + KEY: = No turbidity (No growth), o* = MBC/MFC + = Turbid (Growth) Spectra results The structure of compound EN1 was elucidated using Nuclear Magnetic Resonance Spectroscopy (NMR), 1-DNMR and 2-DNMR and also by comparing the obtained data with already existing literature. The results obtained are as shown in the table. H1NMR: 7.2401, 5.2346, 3.2100, 3.2021, 3.1911, 3.1836, 2.3241, 2.1768, 2.1585, 2.0742, 2.0181, 2.0023, 1.9942, 1.9793, 1.9727, 1.9067, 1.9012, 1.8923, 1.8865, 1.8621, 1.8455,1.8379, 1.8227, 1.7276, 1.7067, 1.6857, 1.6669, 1.6529, 1.6353, 1.6302, 1.6127, 1.5952, 1.5890, 1.5669,1.5463, 1.5230,1.4987,1.4836,1.4667,1.4579,1.4402,1.4276,1.3730,1.3429, 1.3195, 1.2990, 1.2780, 1.2630, 1.2329,1.1813,1.1514,1.1169,1.0870, 1.0639,1.0316, 1.0195,0.9989, 0.9759, 0.9681, 0.9404, 0.9322, 0.9217, 0.9088, 0.8927, 0.8841, 0.8680, 0.8591,0.8450 0.8341 13CNMR: 137.9624, 125.9002, 79.0731, 77.2169, 77.0054, 76.7938, 55.2568, 52.7408, 47.9239, 47.5759, 42.0388, 39.5194, 39.0811, 38.8495, 38.7715, 38.6441, 37.0200, 36.7105, 33.0001, 30.6267, 29.6969, 29.3557, 28.1492, 28.0359, 27.2530, 24.2027, 23.5770, 23.3098, 21.1608, 18.3150,17.0954, 16.9846, 15.5986, 15.4777. The 13C NMR spectrum (Figure 3) showed thirty (30) major recognizable carbon signals, eight methyl groups at ÃŽ ´37.0200 (C-22), 28.0359 (C-23), 15.5986 (C-24), 15.4777 (C-25), 16.9846 (C-26), 24.2027 (C-27), 17.0954 (C-28), 28.1492 (C-29), 23.3098 (C-30) and a secondary hydroxyl bearing carbon 79.0731 at (C-3). It also showed some recognizable signals at ÃŽ ´ 125.9002 and 137.9624 ppm which is assignable to the double bond at C-12 and C-13. In addition, ten methylene groups, eight methyl groups , six quaternary carbons atoms from DEPT experiment were observed. The chemical shift at ÃŽ ´137.962, 125.9002 which are C-12 and C-13 and the consistent flow of methyl groups from C-23 to C-30 were characteristic peaks for a ÃŽ ²- Amyrin type of skeleton [11] and [12]. 1HNMR, 13C NMR and DEPTH spectra of the proposed compound ÃŽ ²-amyrin Figure1: 1HNMR Figure 2 : 13C NMR Figure 3: DEPT Table 4: Comparison of 13C NMR spectrum data of ÃŽ ²-amyrin Obtained from the Aerial parts of M. barteri with literature Carbon Position ÃŽ ´13CNM ÃŽ ´ppm ÃŽ ²-amyrin, Experimental ÃŽ ´13CNMR ÃŽ ´ppm ÃŽ ²-amyrin, Literature [12] ÃŽ ´13CNMR ÃŽ ´ppm ÃŽ ²-amyrin, Literature [11] 1 38.8495 38.7 37.3 2 27.2530 27.2 28.28 3 79.0731 79.3 71.85 4 38.7715 38.5 37.30 5 55.2568 55.1 36.81 6 18.3150 18.6 21.12 7 38.6441 32.4 42.35 8 39.5194 39.8 45.90 9 47.9239 47.6 50.18 10 36.7105 36.9 36.55 11 23.5770 23.6 24.33 12 125.9002 121.7 121.70 13 137.9624 145.2 140.81 14 42.0388 41.7 44.36 15 29.3557 26.2 26.13 16 21.1608 26.1 23.11 17 29.6969 32.6 48.48 18 47.5759 47.2 45.89 19 39.0811 46.8 39.82 20 30.6267 31.0 36.49 21 33.0001 34.7 34.00 22 37.0200 37.1 31.71 23 28.0359 28.0 29.3 24 15.5986 15.4 19.84 25 15.4777 15.4 19.10 26 16.9846 16.8 18.90 27 24.2027 25. 9 18.30 28 17.0954 28.4 19.20 29 28.1492 33.8 36.18 30 23.3098 23.7 19.42 Proposed structure EN1-(C30H50O, 426.7 g/mol) Name: ÃŽ ²-Amyrin or ÃŽ ²-Amyrenol Conclusion The isolation of ÃŽ ²-Amyrin from the aerial plant parts of M. barteri, whose bioactivity was established from this work by its zone of inhibition, is comparable to the drugs of Ciprofloxacin, Fluconazole and Fulcin. This justifies why the plant really serves as a general purpose antibiotic in traditional medicine in our society. The preliminary phytochemical screening also shows that the ethylacetate extract contains other classes of compounds that can be further isolated and tested for microbial activities and this can lead to chains of discoveries as the quest of precursors for modern drugs are continually on course.

Computer Changes and their Impact on Management :: essays research papers

Computer Changes and their Impact on Management The world of computers is a field that is not only very young, but is also rapidly changing. In one lifetime, computers have evolved from a multimillion dollar unit that filled entire buildings, to a few hundred dollar personal computer that fits on a desktop with more power than its predecessor. How does this change in cost, size and power affect our management decisions? To look at these areas, it should be understood that each characteristic not only makes a significant impact for management, but is enhanced by the other two changes.   Ã‚  Ã‚  Ã‚  Ã‚  The reduction in computer costs over time has the obvious implications of reduced overhead costs for a company’s management and possibly a smaller budget requirement for the information technologies department. Closer inspection of the lower computer costs shows that the reduced overhead can have a number of implications dependant on management decisions. One decision would be to show an increased profit on the end product’s margins. This makes stockholders very happy. Management could also decide to provide more computer technology for the company without an increase in budget, or provide hardware with less compromise in functionality. The additional money could be used for additional specialty computer hardware that would not normally be purchased. A company would have to research whether the increase in computer technology would be able to provide the necessary competitive advantages or productivity increases. Management might also decide to take an exhaustive look at the entire company and its business process’ to determine if other areas might use the money for upgrades as well. These upgrades can be in the manufacturing process, research and development, sales, or in manufacturing to name a few. The reduction in cost also impacts the reduction in size of the computer hardware which has been getting smaller and smaller. As the prices of computers have gone down, the manufacturers have been able to reduce the size of the computers dramatically at the same time. Management originally provided a computer that was very large with terminals provided to the user as an interface. Today we have a personal computer on almost every desk top. These units require less space allowing management the freedom to determine whether they wish to put a unit on every desktop. With the smaller space requirements and the changes in the hardware itself, management also has less impact to their facility requirements.

Small Objects: a Literary Analysis of Lullabies for Little Criminals

Small Objects: A Literary Analysis of â€Å"Lullabies for Little Criminals† In â€Å"Lullabies for Little Criminals,† there are many small objects that are relevant to Baby’s life. Objects can have remarkably profound effects on a person’s life, whether they are of sentimental value or another form of personal meaning, they have an impact on us. An object can mean many things to different people. An abandoned doll in a trash bin could be seen as old and ugly to an average person, but to the person who originally owned the doll; it could have been particularly special.In the novel, Heather O’Neil illustrates the effects of such objects on Baby and their symbolic meaning. In â€Å"Lullabies for Little Criminals,† there are three objects that represent Baby’s growth and change throughout the novel: the ragdoll, the knee- high socks, and the toy mice. When introducing her new friend Lauren to her room, Baby reflects on her rag doll,  "It was a doll that my mother had bought for me when she was pregnant . . . The doll also made me feel sweet inside, too, because it made me feel that at some point, even before I existed, I had been loved† (O’Neill 97-98).This illustrates Baby’s longing for a loving mother figure, which is a reasonable expectation from a 12 year old girl. Loving care is a critical need of any child. Baby does not have that feeling of being loved; therefore, she finds comfort in the fact that she was once loved. The doll is also representative of her current state of mind. Such as her wishes to be normal: have normal friends, normal parents, normal family; a normal life. When Jules destroys Baby’s rag doll out of anger, it is symbolic of a lost childhood.Her last reminder of the love her mother had for her had been torn away. Baby says, â€Å"Now I was nothing, a real nobody (O’Neill 119). The destruction of her doll meant that her sense of belonging, that she was once part of a family, was now gone. She seems as if she is being pulled into adolescence without having any real sense of true childhood. When the character Alphonse begins to take an interest in Baby, his first attempt to make Baby take an interest in him is to give her a gift. The gift is a pair of dainty knee-high socks.Baby wants nice things, and these socks were, according to baby, â€Å"the first pretty things I’d ever owned† (O’Neill 155). This gift is exhilarating for her. It is an acknowledgment from someone who believed she was attractive, especially coming from Alphonse. According the others in the neighborhood, it was known that Alphonse only looked out for good looking women, so if he took notice of someone, it meant something (O’Neill 148). It is particularly clear to the reader that Alphonse likely has ulterior motives, but to baby Alphonse is simply someone who likes her.The socks remind Baby that she is pretty, and she is proud of the fa ct that an older man found her attractive. In turn, this causes baby to trust Alphonse and fall for his manipulation, leading towards what could be a life of prostitution. The socks are symbolic of Baby being forced into adulthood. Baby is still young and naive; she has no way of understanding what Alphonse is attempting to do. Therefore, the socks may also represent Baby’s vulnerability and lack of ability to judge character.At this point in the novel, Baby has not had a proper childhood or even learned any of what it means to be a teenage person, and now she is already being pushed into adulthood. After Alphonse dies in the hotel room, Baby does not know what to do. She is lost without an adult. She realizes, â€Å"Even though I was making all the money, it seemed that since he was the adult, he was the only one who could get us a place to stay and food to eat† (O’Neill 309). It is clear that Baby is still mentally and physically a child, but has taken on the role of an adult.Since there is no longer an adult in her life, she is immediately overwhelmed. She does not think seeing Jules is a good idea (O’Neill 311), however, for some reason she is compelled to go to the shelter in which he is residing in. It seems that Baby’s situation may be hopeless. Baby’s arrival to the Mission seems to be the crucial turning point in the novel. She is presented with a family of toy mice, which baby believes to be â€Å"by far, the best gift I’d ever gotten† (O’Neill 317). An innocent and beautiful gift, which is exactly what Baby needs, something she has not had in an exceptionally long time.The mice are given along with the knowledge that she will be living Jules’s cousin from now on. The mice represent the possibilities of positive change in Baby’s life. They are symbolic of hope; the possibility that she will find what she needs. In â€Å"Lullabies for Little Criminals,† the use of obje cts to create symbolic meaning is abundantly evident and illustrates the roles Baby assumes as the novel progresses. She does not experience growing up in the same way most children do. She seems to go from child, to adolescent teen, to adult, in approximately a year.The roles she assumes show her growth in a manner that is unnatural to the reader, and the use of symbolism to show how this process is forced onto her is profound. The symbolism of the toy mice representing change shows Baby’s final step towards becoming a â€Å"normal† person. The author’s use of this immediately after the seemingly darkest point in the novel serves to create an emotional uplift for the reader; ultimately allowing the reader to realize that the character is going to be alright. The narrator states, â€Å"Then Janine stepped over to me and squeezed me hard.I could feel my heart beating when she hugged me against her, but now it felt fine . . . Her big blue eyes looked just like Ju les’s, and I guess mine too. Her green winter jacket smelled like rain† (O’Neill 330). The final paragraph of the novel shows that Baby will likely get exactly what she wanted: Love, a caring person, family, belonging, and most importantly, the opportunity to find herself. Work Cited O’Neill, Heather. Lullabies for Little Criminals. 1st ed. Toronto: Harper Perennial, 2006. Print

Free Essays on Realizing Blake’s Vision

Blake’s poems explore a world that he envisions as the â€Å"two contrary states of the human soul† (Norton 37). He uses two books of poems, the Songs of Experience and the Songs of Innocence, to represent his different points of view. In these books, Blake uses companion poems to mix related content and imagery to represent his viewpoints of innocence and experience. â€Å"The Ecchoing Green† and â€Å"London† are two particular poems from these books that can be considered companion poems. Clearly Blake’s double vision about the world is satisfied by these two poems. They are linked to each other by their content and imagery and the establishment of opposing tones and settings. â€Å"The Ecchoing Green† from Blake’s Songs of Innocence is the first from this set of companion poems. Blake’s innocence poems are typically of a livelier and happier tone. In the first stanza, happiness and innocence are established by the sun rising, the skies being happy, and the spring being welcomed by birds singing and by other pleasant sounds (1-8). Carefree-ness is portrayed particularly in the next stanza by Old John laughing amongst the old folk as they all reminisce and laugh at their youthful past (11-20). Blake’s setting in the green field also portrays a place of carefree fun, a place to play loud sports and is presented as a more casual type of atmosphere. (The lively ecchoing green field is comparable to a present day community recreational park.) The most important images in the poem however are the children and the sense of family in the final stanza. After the children have wearied and the day draws to a close, their mother s and their â€Å"many sisters and brothers† are all compared to â€Å"birds in their nest,† who â€Å"Are ready for rest† (27-28). This comparison represents a peaceful end of the day with the children safely returning home to their caring mothers and families. The imagery of nature in this poem gl... Free Essays on Realizing Blake’s Vision Free Essays on Realizing Blake’s Vision Blake’s poems explore a world that he envisions as the â€Å"two contrary states of the human soul† (Norton 37). He uses two books of poems, the Songs of Experience and the Songs of Innocence, to represent his different points of view. In these books, Blake uses companion poems to mix related content and imagery to represent his viewpoints of innocence and experience. â€Å"The Ecchoing Green† and â€Å"London† are two particular poems from these books that can be considered companion poems. Clearly Blake’s double vision about the world is satisfied by these two poems. They are linked to each other by their content and imagery and the establishment of opposing tones and settings. â€Å"The Ecchoing Green† from Blake’s Songs of Innocence is the first from this set of companion poems. Blake’s innocence poems are typically of a livelier and happier tone. In the first stanza, happiness and innocence are established by the sun rising, the skies being happy, and the spring being welcomed by birds singing and by other pleasant sounds (1-8). Carefree-ness is portrayed particularly in the next stanza by Old John laughing amongst the old folk as they all reminisce and laugh at their youthful past (11-20). Blake’s setting in the green field also portrays a place of carefree fun, a place to play loud sports and is presented as a more casual type of atmosphere. (The lively ecchoing green field is comparable to a present day community recreational park.) The most important images in the poem however are the children and the sense of family in the final stanza. After the children have wearied and the day draws to a close, their mother s and their â€Å"many sisters and brothers† are all compared to â€Å"birds in their nest,† who â€Å"Are ready for rest† (27-28). This comparison represents a peaceful end of the day with the children safely returning home to their caring mothers and families. The imagery of nature in this poem gl...

Free Essays on Methodic Doubt

Methodic Doubt Rene Descartes purposely identifies his work as â€Å"a meditation† and presents his thoughts using the first person. He doubts everything that he has admitted to up until this point because he feels that the senses can be deceiving. The senses, according to Descartes, were bad indicators to what is real because one, there is the chance of error and two; the actual experience of dreaming is misleading. Descartes thought that by doubting everything and anything, he could erase all other knowledge and begin to build from the base up. This idea is like an architects’, being that they must have a solid foundation before they can proceed. His ideas were very systematic, similar to those used by scientists and rational simply because he did not want to just add to things done earlier by others. He began with the determination to doubt all and take nothing for granted. While doing so, he stumbled across one proposition that he could not doubt. And that was his own existence. He stated that after all, the simple act of doubting presupposed something, which was engaging in doubt. This famous proposition is known as â€Å"cogito, ergo sum†: I think, therefore I am. Descartes concluded from this that at least one existential truth existed that we can claim to know. This was, that we, as individual persons, exist as thinking beings. It is on this premise that he attempts to base anything else because he felt that any secure philosophy must have a strong and secure starting point. It is from this point that he attempts to prove the existence of god and other things, which he thinks he can deduce. Following Descartes’ doubts of the senses he begins to doubt mathematics. He states the â€Å"Evil Genius Hypothesis.† This bold new idea states that maybe god is evil and that there is a higher being who likes to deceive others. With that in mind he states that this deception implies two things. First it imp... Free Essays on Methodic Doubt Free Essays on Methodic Doubt Methodic Doubt Rene Descartes purposely identifies his work as â€Å"a meditation† and presents his thoughts using the first person. He doubts everything that he has admitted to up until this point because he feels that the senses can be deceiving. The senses, according to Descartes, were bad indicators to what is real because one, there is the chance of error and two; the actual experience of dreaming is misleading. Descartes thought that by doubting everything and anything, he could erase all other knowledge and begin to build from the base up. This idea is like an architects’, being that they must have a solid foundation before they can proceed. His ideas were very systematic, similar to those used by scientists and rational simply because he did not want to just add to things done earlier by others. He began with the determination to doubt all and take nothing for granted. While doing so, he stumbled across one proposition that he could not doubt. And that was his own existence. He stated that after all, the simple act of doubting presupposed something, which was engaging in doubt. This famous proposition is known as â€Å"cogito, ergo sum†: I think, therefore I am. Descartes concluded from this that at least one existential truth existed that we can claim to know. This was, that we, as individual persons, exist as thinking beings. It is on this premise that he attempts to base anything else because he felt that any secure philosophy must have a strong and secure starting point. It is from this point that he attempts to prove the existence of god and other things, which he thinks he can deduce. Following Descartes’ doubts of the senses he begins to doubt mathematics. He states the â€Å"Evil Genius Hypothesis.† This bold new idea states that maybe god is evil and that there is a higher being who likes to deceive others. With that in mind he states that this deception implies two things. First it imp...

Work and Non-Work Relationship Essay Example | Topics and Well Written Essays - 3000 words

Work and Non-Work Relationship - Essay Example From the discussion it is clear that  for the purposes of explaining the relationship between job responsibilities and family responsibilities, various kinds of conceptual models have been proposed in this regard, and these conceptual models symbolize the diverse viewpoints on how we carry out our respective job responsibilities and family responsibilities. Although, various conceptual models have been introduced for the purpose of explaining the relationship between job responsibilities and family responsibilities, but the five major conceptual models which have been introduced in this regard, includes, segmentation model, spillover model, compensation model, instrumental model and conflict model.  This paper declares that  different methodologies have been used by the researchers for the purpose of assessing the relationship between enhanced work loads of dismissed survivors and their respective work-life balance and work and life contentment. As per a research, employees who face extremely heavy workload, have to face heavy overall role overload as well, and this eventually creates a negative influence on their work-life balance.   Another research was carried out for the purpose of discovering the fact that whether contented employees maintain an effective work-life balance or not, and in this research, the researchers found that flexible functioning approach facilitates employees to stay aggravated, which eventually helps them to accomplish their respective professional and personal goals effectively and efficiently.

How Going Public For Facebook Has Affected The Company Research Paper

How Going Public For Facebook Has Affected The Company - Research Paper Example Since that time, it has seen its users numbers grow to over 900 million users who are active. Handset devices are the majorly used by the Facebook subscribers. As typically anticipated, changing a company’s status from a private to a public status happens to be a commemorative period for the commercial entity holder, as well as, the management. The IPO signifies a reward for periods of thriving practices of the Facebook Company and the affirmative reception by the consumers. Nevertheless, the taking of the Facebook Company happened to highlight the significant weaknesses perhaps as an entity that is not geared up to see through the extra burden of finances and consequently lack of control. This is brought about by the initiation into the market of the stock exchange. As is the norm when preparing a company for an IPO issuance, a company cannot put out the information regarding its finances with any prospective investors or present investors through the quiet period. Therefore, investing in the company comes out blindly, a case that could make the investors lose their finances on the takeover supposing the company is overvalued as is the Facebook Company. In the process of making acquisition briefly after the IPO, since the overvaluation result in the unreasonably investors being buoyant, there is bound to be an overpayment. The challenges the Facebook Company has undergone since transforming its status into being a public company have posed numerous challenges to its undertakings. These challenges have consequently had a bearing effect on the company. Introduction The process of acquiring a public status of a trading company dealing in the stock exchange market from a private entity is not a procedure to be assumed lightly. The procedure is rigorous and takes up a lot of the financial resources, the dedication of management and the company’s time. However, the result of transforming into a public entity is significant as it comes along with an inc rease in the finances together with an increase in the level of awareness by the public. In addition, there is also the increase in the requirements of the legal nature and the corporate responsibility. Facebook Company’s intention of having IPO was essentially become traded in the public domain and consequently become bigger. Therefore, the individuals who put up their finances in the company through purchasing of the shares of the company gain a part of ownership of the company for an opportunity to share in the advantages of the company’s upcoming profits. For the owner of the Facebook Company, part of control he encompasses for the company was given up relative to the percentage of the company put up on sale. On top of being answerable to its shareholders, the Facebook Company has been opened to scrutiny by the government and the public. Nonetheless, the anticipation is that the Facebook Company will be able to generate extra capital resources as its capability to do so have been considerably improved. The general net worth of the company which is necessitated by the selling of the company’s stock, results in the company attaining such capabilities. The thriving of the stock of the company as was expected prior to the IPO was that it would play a part in the decreasing of any ratio of the debt versus the income, consequently assisting in the enhancement of the company’s score on its credit. Therefore, supposing the Facebook Company after attaining the public trading company status had successful stock provision, lenders can subsequently is found easily. This is because of the enhanced rating of credit